Thyroglobulin (Thyroidal Cell Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC|
|Reactivity||Human, Mouse, Rat|
|Predicted||Human, Mouse, Rat|
|Isotype||IgG1, kappa + IgG1, kappa|
|Calculated MW||660kDa (Dimeric Form)|
|Other Names||Thyroglobulin, Tg, TG|
|Target/Specificity||Human thyroid follicular cells|
|Application Note||Flow Cytometry : 0.5-1.0ug/million cells|
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (formalin-fixed) (0.5-1.0 µg/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Thyroglobulin (Thyroidal Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).|
|Tissue Location||Thyroid gland specific.|
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Provided below are standard protocols that you may find useful for product applications.
Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.
1. Ossendorp FA, et. al. Journal of Immunological Methods, 1989, 120(2):191-200.
2. Bellet, D, et al. J Clin Endocrin Metab 1983;56:530-533
3. Heffess CS et al. Cancer. 2002;95(9):1869-78
4. Judkins AR et al. Hum Pathol. 1999;30(11):1373-6
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