Thymidylate Synthase (5-FU Resistance Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Other Names||Thymidylate synthase, TS, TSase, TYMS, TS|
|Target/Specificity||Recombinant human thymidylate synthase|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.25-0.5ug/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Thymidylate Synthase (5-FU Resistance Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.|
|Cellular Location||Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 126.96.36.199). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
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3. Johnston PG, et. al. Biochemical Pharmacology, 1993, 45(12):2483-6.
4. Johnston PG, et. al. Journal of Clinical Oncology, 1994, 12(12):2640-7.
5. Johnston PG, et. al. Cancer Research, 1995, 55(7):1407-12.
6. Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13.
7. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31.
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