|Application ||WB, IHC, IF, FC|
|Target/Specificity||Human rheumatoid synovial T cell line (ST-1)|
|Application Note||Biological Blockade (Partially blocks laminin binding) (Order Ab without Azide);|
ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) : 0.5-1.0 µg/ml for 30 minutes at RT.
|Format||0.5 ml of Bioreactor Concentrate with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CDw60 Antibody [Clone UM4D4] is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
1. Fox DA et. al. in Knapp W (ed) Leucocyte Typing IV 1989; pp364-366 (Oxford University Press, London).
2. Fox DA et. al. Immunol Invest 30(2):67-85, 2001.
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