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Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide AH10356
    Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Reactivity Human
Predicted Chicken, Cat, Dog, Goat, Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names VM452
Calculated MW 57-60kDa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity Human vimentin recombinant protein
Application Note Flow Cytometry : 5ul (0.2ug) per test per one million cells .
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.


1. Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10356
(40 western blots)
Availability: 2-3 days
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