Cyclin D1 (G1-Cyclin & Mantle Cell Marker)Mouse Monoclonal Antibody [Clone DCS-6]
Purified Mouse Monoclonal Antibody
|Application ||IF, FC|
|Other Accession||P25322, P39948 (Rat)|
|Reactivity||Human, Mouse, Rat, Monkey|
|Other Names||B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene|
|Target/Specificity||Human recombinant full length cyclin D1 protein|
|Application Note||low Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT)； (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)； Optimal dilution for a specific application should be determined.|
|Format||0.5ml at 100ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cyclin D1 (G1-Cyclin & Mantle Cell Marker)Mouse Monoclonal Antibody [Clone DCS-6] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.|
|Cellular Location||Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members|
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Provided below are standard protocols that you may find useful for product applications.
1. Lukas J, et. al. Oncogene, 1994, 9(3):707-18.
2. Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7.
3. Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45.
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