CD63 (Late Endosomes Marker)Mouse Monoclonal Antibody [Clone NKI/C3]
Purified Mouse Monoclonal Antibody
|Application ||IF, FC|
|Calculated MW||26kDa (core protein); 30-60kDa (glycosylated)|
|Other Names||gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30|
|Target/Specificity||Smooth plasma membrane fraction of MeWo cells|
|Application Note||Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT); (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.|
|Format||0.5ml at 100ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD63 (Late Endosomes Marker)Mouse Monoclonal Antibody [Clone NKI/C3] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.|
|Cellular Location||Cell membrane; Multi- pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi- pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, exosome. Cell surface. Note=Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre- melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903)|
|Tissue Location||Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages|
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Provided below are standard protocols that you may find useful for product applications.
1. C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985.
2. AA Palmer et al., Pathology 17: 335-339, 1985.
3. EC Hagen et al., Histopathology 10: 689-700, 1986.
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