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Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody [Clone AE-1/AE-3]

Purified Mouse Monoclonal Antibody

     
  •  - Cytokeratin, pan (Epithelial Marker)
Mouse Monoclonal Antibody [Clone AE-1/AE-3] AH10374-50
    Flow Cytometric analysis of human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin MAb (AE-1/AE-3).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Primary Accession Q7Z794
Other Accession Q01546
Reactivity Human, Mouse, Rat, Rabbit, Monkey, Chicken, Bovine, Dog
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa + IgG1, kappa
Clone Names AE-1/AE-3
Calculated MW 40-67kDa
Additional Information
Gene ID 374454
Other Names K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39
Target/Specificity Human epidermal keratin
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Format 0.5ml at 100ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsCytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody [Clone AE-1/AE-3] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT77
Synonyms KRT1B
Tissue Location Expressed exclusively in skin.
Research Areas
Citations (0)
citation

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References

1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
2. Tseng SCG et. al. Cell 1982; 30361.

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$ 429.00
Cat# AH10374-50
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