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MUC1 / EMA / CD227 (Epithelial Marker) Antibody - Without BSA and Azide

Mouse Monoclonal Antibody [Clone SPM533 ]

  • IHC -  MUC1 / EMA / CD227 (Epithelial Marker) Antibody - Without BSA and Azide AH10602
    Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (SPM533).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15941
Other Accession 4582, 89603
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG2a, kappa
Clone Names SPM533
Calculated MW 265-400kDa
Additional Information
Gene ID 4582
Other Names Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions MUC1 / EMA / CD227 (Epithelial Marker) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MUC1
Synonyms PUM
Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Cellular Location Apical cell membrane; Single- pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions Isoform Y: Secreted. Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus
Tissue Location Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.
Research Areas
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In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This protein is proteolytically cleaved into α and β subunits that form a heterodimeric complex. The N-terminal α subunit and the C-terminal β subunit. . The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The β subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.


Cordell J et al. 1985. Br J Cancer 52(3):347-54. | Heyderman E et al. 1985. Br J Cancer 52(3):355-61

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$ 354.00
$ 484.00
Cat# AH10602
(40 western blots)
Availability: 2-3 days
Bulk Size
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