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MyoD1 (Rhabdomyosarcoma Marker) Antibody - Without BSA

Mouse Monoclonal Antibody [Clone SPM427 ]

  • IHC -  MyoD1 (Rhabdomyosarcoma Marker) Antibody - Without BSA AH10618
    Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Monoclonal Antibody (SPM427)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15172
Other Accession 4654, 181768
Reactivity Human, Mouse, Rat, Chicken
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names SPM427
Calculated MW 45kDa
Additional Information
Gene ID 4654
Other Names Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions MyoD1 (Rhabdomyosarcoma Marker) Antibody - Without BSA is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYOD1
Synonyms BHLHC1, MYF3, MYOD
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilmā€™s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewingā€™s sarcomas and alveolar soft part sarcomas.


Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41. | Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. | Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11

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$ 324.00
$ 484.00
Cat# AH10618
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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