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PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM350 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | P12004 |
| Other Accession | 5111, 147433, 728886 |
| Reactivity | Human, Mouse, Rat, Zebrafish, Monkey, Pig, Chicken, Yeast, Drosophila |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2a, kappa |
| Clone Names | SPM350 |
| Calculated MW | 36kDa |
| Gene ID | 5111 |
|---|---|
| Other Names | Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PCNA |
|---|---|
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737). |
| Cellular Location | Nucleus Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
References
Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9. | Hall PA et al. 1990. J. Pathol. 162(4):285-94. | Landberg G & Roos G. 1991. Cancer Res. 51 (17):4570-4. | Woods AL et al. 1991. Histopathol. 19(1):21-7 | Yu,CC. et al. 1991. Histopathol. 19(1):29-33
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