CD71 / Transferrin Receptor (TFRC) Antibody - Without BSA and Azide
Mouse Monoclonal Antibody [Clone 66IG10 ]
|Application ||IF, FC|
|Other Accession||7037, 529618|
|Isotype||Mouse / IgG1, kappa|
|Calculated MW||85-95kDa (monomer); 190kDa (dimer)|
|Other Names||Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, CD71, Transferrin receptor protein 1, serum form, sTfR, TFRC|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD71 / Transferrin Receptor (TFRC) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Van de Rijna M, Geurts van Kessel AHM, Kroezen V, van Agthoven AJ, Verstijnen K, Terhorst C, Hilgers J: Cytogenet Cell Genet 1983;36:525-531. | Oudermans et al. Cancer, 1986; 58:1252. | K. Moolenaar et al. Cancer research 50,1102-1106, 1990
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