TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM357 ]
|Application ||IF, FC|
|Other Accession||7039, 170009|
|Reactivity||Human, Mouse, Rat, Cow|
|Isotype||Mouse / IgG1, kappa|
|Other Names||Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.|
|Cellular Location||Transforming growth factor alpha: Secreted, extracellular space|
|Tissue Location||Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines|
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Provided below are standard protocols that you may find useful for product applications.
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. | Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. | Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. | Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3 |
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