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Vimentin (Mesenchymal Cell Marker) Antibody - Without BSA and Azide

Mouse Monoclonal Antibody [Clone SPM576 ]

  • IHC -  Vimentin (Mesenchymal Cell Marker) Antibody - Without BSA and Azide AH10810
    Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (SPM576).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Other Accession 7431, 455493
Reactivity Human, Pig, Chicken, Goat, Cow, Dog, Cat
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names SPM576
Calculated MW 57-60kDa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Vimentin (Mesenchymal Cell Marker) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPā€™s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.ĀAnti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.


Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

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$ 354.00
$ 484.00
Cat# AH10810
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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