Cyclin B1 (G2- & M-phase Cyclin) Antibody - Without BSA and Azide
Mouse Monoclonal Antibody [Clone V92.1 ]
|Application ||IF, FC, IP|
|Other Accession||891, 23960|
|Reactivity||Human, Mouse, Hamster|
|Isotype||Mouse / IgG1, kappa|
|Other Names||G2/mitotic-specific cyclin-B1, CCNB1, CCNB|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cyclin B1 (G2- & M-phase Cyclin) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for the control of the cell cycle at the G2/M (mitosis) transition.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Garner AP et al. Oncogene 21:8089-104 (2002) | Minemoto Y et al. Exp Cell Res 262:37-48 (2001
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