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Biotin (Vitamin B7 or Vitamin H) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM375 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | SPM375 |
| Calculated MW | 244Da |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
|---|---|
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Biotin (Vitamin B7 or Vitamin H) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
References
Uccellini MB, et al. 2008. J. Immunol. 181:5875. | Green NM, et al. 2012. J Biol Chem. 287:3978
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