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Cytokeratin, pan (Epithelial Marker) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone SPM115 + SPM116 ]

     
  • IHC -  Cytokeratin, pan (Epithelial Marker) Antibody - With BSA and Azide AH10955-20
    Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (SPM115 + SPM116).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC
Primary Accession Q7Z794
Other Accession 374454 (KRT77), 51350 (KRT76), 334989 (KRT77), 654392 (KRT76), Q01546
Reactivity Human, Mouse, Rat, Rabbit, Monkey, Chicken, Cow, Dog
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG's
Clone Names SPM115 + SPM116
Calculated MW 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
Additional Information
Gene ID 374454
Other Names Keratin, type II cytoskeletal 1b, Cytokeratin-1B, CK-1B, Keratin-77, K77, Type-II keratin Kb39, KRT77, KRT1B
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Cytokeratin, pan (Epithelial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT77
Synonyms KRT1B
Tissue Location Expressed exclusively in skin.
Research Areas
Citations (0)
citation

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Background

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

References

Tseng SCG et. al. Cell 1982; 30361. | Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8

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$ 219.00
$ 499.00
Cat# AH10955-20
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