Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - Without BSA and Azide
Mouse Monoclonal Antibody [Clone AE-3 ]
|Application ||WB, IHC-P, IF, FC|
|Other Accession||51350, 654392|
|Reactivity||Human, Mouse, Rat, Rabbit, Monkey, Chicken, Cow, Dog|
|Isotype||Mouse / IgG1, kappa|
|Other Names||Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, CK-2P, K2P, Keratin-76, K76, Type-II keratin Kb9, KRT76, KRT2B, KRT2P|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably contributes to terminal cornification.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. | Tseng SCG et. al. Cell 1982; 30361. |
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