|Application ||IF, FC|
|Other Accession||10507, 494406|
|Isotype||Mouse / IgM, kappa|
|Calculated MW||50kDa (Monomer)|
|Other Names||Semaphorin-4D, A8, BB18, GR3, CD100, SEMA4D, C9orf164, CD100, SEMAJ|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD100 (Semaphorin-4D) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C9orf164, CD100, SEMAJ|
|Function||Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.|
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Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997. | Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995. | Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276. | Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262. | Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268. | Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520. | Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-1178
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