Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody
Mouse Monoclonal Antibody [Clone TOP1MT/488 ]
|Application ||IHC, IF, FC|
|Other Accession||116447, 528574|
|Isotype||Mouse / IgG2b, kappa|
|Other Names||DNA topoisomerase I, mitochondrial, TOP1mt, 18.104.22.168, TOP1MT|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).|
|Tissue Location||Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.|
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Provided below are standard protocols that you may find useful for product applications.
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Zhang, H., Barcel�, J.M., Lee, B., Kohlhagen, G., Zimonjic, D.B., Popescu, N.C. and Pommier, Y. 2001. Human mitochondrial topoisomerase I. Proc. Natl. Acad. Sci. USA 98: 10608-10613. | Zhang, H., Meng, L.H. and Pommier, Y. 2007. Mitochondrial topoisomerases and alternative splicing of the human TOP1mt gene. Biochimie 89: 474-481
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