|Application ||WB, IHC, IF, FC|
|Other Accession||26271, 520506|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||F-box only protein 5, Early mitotic inhibitor 1, FBXO5, EMI1, FBX5|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||EMI1 (Early Mitotic Inhibitor-1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Reimann, J.D., et al. 2001. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 105: 645-655. |
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