|Application ||IHC, IF, FC|
|Other Accession||3383, 643447|
|Isotype||Mouse / IgG2b, kappa|
|Clone Names||W-CAM-1; same as Wehi-CAM-1 or 1H4|
|Other Names||Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD54 / ICAM-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Boyd AW et. al. Blood, 1989, 73(7):1896-903. | Boyd AW et. al. Proceedings of the National Academy of Sciences, 1988, 85(9):3095 | Wawryk et al. J Clin Pathol 44, 497-501 (1991). | Fecondo et al., Proc. Nat. Acad. Sci. 88(7), 28792882, (1991)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.