|Application ||IHC, IF, FC|
|Other Accession||3385, 354563|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD50 / ICAM-3 Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
Vilella R, et. al. Tissue Antigens, 1990, 36(5):203-10. | Pino-Otin MR, et. al. Journal of Immunology, 1995, 154(6):3015-24. | Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press, 1989. | Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, 1993
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