|Application ||IHC, IF, FC|
|Other Accession||4267, 653349|
|Isotype||Mouse / IgG's|
|Clone Names||12E7 + MIC2/877|
|Other Names||CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD99 / MIC2 (Ewing's Sarcoma Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MIC2, MIC2X, MIC2Y|
|Function||Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).ĀAlthough its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.ĀCD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewingā€™s sarcoma cells and primitive peripheral neuroectodermal tumors.
Levy R, Dilley J, Fox RI, Warnke R. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. PNAS USA 1979;76(12):6552-
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