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>   home   >   Products   >   Primary Antibodies   >    Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide   

Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone NCL/902 ]

     
  • WB -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Western Blot of A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • IHC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • FC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Flow Cytometry of Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • FC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    HeLa Cells stained with AF488 labeled Nucleolin Monoclonal Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.
    detail
  • FC -  Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide AH11978-20
    Flow Cytometry of Human Nucleolin Ag on Jurkat Cells. Grey: Isotype Control; Green: FITC-labeled; Purple: APC-Labeled; Turcoise: AF700-Labeled Nucleolin Monoclonal Antibody (NCL/902).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, FC
Primary Accession P19338
Other Accession 4691, 79110
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names NCL/902
Calculated MW 76kDa
Additional Information
Gene ID 4691
Other Names Nucleolin, Protein C23, NCL
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Nucleolin (Marker of Human Cells) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCL
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG- 3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Citations (0)
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Background

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.ĀThis MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

References

Fujiki H, Watanabe T, Suganuma M. Cell-surface nucleolin acts as a central mediator for carcinogenic, anti-carcinogenic, and disease-related ligands. J Cancer Res Clin Oncol. 2014;140(5):689-99. | Qiu W, Zhou F, Zhang Q, Sun X, Shi X, Liang Y, Wang X, Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS. 2013;121(10):919-25. |

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$ 219.00
$ 499.00
Cat# AH11978-20
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