PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody
Mouse Monoclonal Antibody [Clone PCNA/694 ]
|Application ||WB, IHC, IF, FC|
|Other Accession||5111, 147433, 728886|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.|
|Cellular Location||Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.|
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Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9. | Hall PA et al. 1990. J. Pathol. 162(4):285-94
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