Cytochrome C (Mitochondrial Marker) Antibody - Without BSA and Azide
Mouse Monoclonal Antibody [Clone 7H8.2C12 ]
|Application ||WB, IHC, IF, FC|
|Other Accession||54205, 437060|
|Reactivity||Human, Mouse, Rat, Horse, Dog|
|Isotype||Mouse / IgG2b, kappa|
|Other Names||Cytochrome c, CYCS, CYC|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytochrome C (Mitochondrial Marker) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Cellular Location||Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane|
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It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Goshorn SG, E Retzel, and R Jemmerson. Common Structural Features among Monoclonal Antibodies Binding the Same Antigenic Region of Cytochrome c. J Biol Chem 266:2134-2142 (1991). |
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