CD45RA (Leucocyte Marker) Antibody - Without BSA and Azide
Mouse Monoclonal Antibody [Clone PTPRC/818 ]
|Application ||IHC, IF, FC|
|Other Accession||5788, 654514|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 22.214.171.124, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD45RA (Leucocyte Marker) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts|
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Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
West, K.P., et al. 1986. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections. J. Pathol. 150: 89-101. | Streuli, M., et al. 1987. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J. Exp. Med. 166: 1548-1566
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