|Application ||IHC, IF, FC|
|Other Accession||5788, 654514|
|Isotype||Mouse / IgG1, kappa + Mouse / IgG1, kappa|
|Clone Names||SPM569 + SPM570|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 188.8.131.52, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD45 / LCA (Leucocyte Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts|
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Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62. | Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5
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