|Application ||IHC-P, IF, FC|
|Other Accession||6876, 410977|
|Reactivity||Human, Mouse, Rabbit, Pig, Cow|
|Isotype||Mouse / IgG1, kappa|
|Other Names||Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, TAGLN, SM22, WS3-10|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Transgelin (SM22-alpha) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
Shapland, C., et al. 1993. Purification and properties of transgelin: a transformation and shape change sensitive Actin-gelling protein. J. Cell Biol. 121: 1065-1073
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