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TGF-alpha (Transforming Growth Factor alpha) Antibody - Without BSA and Azide

Mouse Monoclonal Antibody [Clone TGFA/1119 ]

     
  • IHC -  TGF-alpha (Transforming Growth Factor alpha) Antibody - Without BSA and Azide AH12404-100
    Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Monoclonal Antibody (TGFA/1119)
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC
Primary Accession P01135
Other Accession 7039, 170009
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names TGFA/1119
Calculated MW ~6kDa
Additional Information
Other Names Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions TGF-alpha (Transforming Growth Factor alpha) Antibody - Without BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFA
Function TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Cellular Location Transforming growth factor alpha: Secreted, extracellular space
Tissue Location Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines EMBL; K03222; AAA61159.1; -; mRNA EMBL; M31172; AAA61157.1; -; mRNA EMBL; X70340; CAA49806.1; -; mRNA EMBL; AF123243; AAF13491.1; -; Genomic_DNA EMBL; AF123238; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123239; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123240; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123241; AAF13491.1; JOINED; Genomic_DNA EMBL; AF123242; AAF13491.1; JOINED; Genomic_DNA EMBL; AY325886; AAP97822.2; -; Genomic_DNA EMBL; AY325885; AAP97822.2; JOINED; Genomic_DNA EMBL; AY326405; AAP97822.2; JOINED; Genomic_DNA EMBL; AY327131; AAP97822.2; JOINED; Genomic_DNA EMBL; AY327132; AAP97822.2; JOINED; Genomic_DNA EMBL; AY329368; AAP97822.2; JOINED; Genomic_DNA EMBL; AF149096; AAF05089.1; -; mRNA EMBL; AF149097; AAF05090.1; -; mRNA EMBL; AF149098; AAF05091.1; -; mRNA EMBL; BT006833; AAP35479.1; -; mRNA EMBL; AK290151; BAF82840.1; -; mRNA EMBL; AC005234; AAY14793.1; -; Genomic_DNA EMBL; AC017084; AAY14705.1; -; Genomic_DNA EMBL; CH471053; EAW99810.1; -; Genomic_DNA EMBL; CH471053; EAW99812.1; -; Genomic_DNA EMBL; BC005308; AAH05308.1; -; mRNA EMBL; M22440; AAA52530.1; -; Genomic_DNA EMBL; AF075584; AAD12238.1; -; Genomic_DNA EMBL; AF075583; AAD12238.1; JOINED; Genomic_DNA CCDS; CCDS1905.1; -. [P01135-1] CCDS; CCDS46316.1; -. [P01135-2] PIR; JN0876; WFHU1 RefSeq; NP_001093161.1; NM_001099691.2. [P01135-2] RefSeq; NP_001295087.1; NM_001308158.1 RefSeq; NP_001295088.1; NM_001308159.1 RefSeq; NP_003227.1; NM_003236.3. [P01135-1] UniGene; Hs.170009; - UniGene; Hs.628298; - PDB; 1GK5; NMR; -; A=83-89 PDB; 1MOX; X-ray; 2.50 A; C/D=40-89 PDB; 1YUF; NMR; -; A=40-89 PDB; 1YUG; NMR; -; A=40-89 PDB; 2TGF; NMR; -; A=40-89 PDB; 3E50; X-ray; 2.30 A; C/D=40-89 PDB; 3TGF; NMR; -; A=40-89 PDB; 4TGF; NMR; -; A=40-89 PDB; 5KN5; X-ray; 2.80 A; C/F=49-88 PDBsum; 1GK5; - PDBsum; 1MOX; - PDBsum; 1YUF; - PDBsum; 1YUG; - PDBsum; 2TGF; - PDBsum; 3E50; - PDBsum; 3TGF; - PDBsum; 4TGF; - PDBsum; 5KN5; - ProteinModelPortal; P01135; - SMR; P01135; - BioGrid; 112897; 68 DIP; DIP-5765N; - IntAct; P01135; 10 MINT; P01135; - STRING; 9606.ENSP00000295400; - SwissPalm; P01135; - BioMuta; TGFA; - DMDM; 135689; - PaxDb; P01135; - PeptideAtlas; P01135; - PRIDE; P01135; - ProteomicsDB; 51332; - ProteomicsDB; 51333; -. [P01135-2] ProteomicsDB; 51334; -. [P01135-3] ProteomicsDB; 51335; -. [P01135-4] ProteomicsDB; 51336; -. [P01135-5] DNASU; 7039; - Ensembl; ENST00000295400; ENSP00000295400; ENSG00000163235. [P01135-1] Ensembl; ENST00000418333; ENSP00000404099; ENSG00000163235. [P01135-2] Ensembl; ENST00000445399; ENSP00000387493; ENSG00000163235. [P01135-3] GeneID; 7039; - KEGG; hsa:7039; - UCSC; uc002sgs.4; human. [P01135-1] CTD; 7039; - DisGeNET; 7039; - EuPathDB; HostDB:ENSG00000163235.15; - GeneCards; TGFA; - HGNC; HGNC:11765; TGFA HPA; HPA042297; - MalaCards; TGFA; - MIM; 190170; gene neXtProt; NX_P01135; - OpenTargets; ENSG00000163235; - Orphanet; 1991; Cleft lip with or without cleft palate Orphanet; 2227; Hypodontia Orphanet; 99798; Oligodontia PharmGKB; PA36480; - eggNOG; ENOG410IVW5; Eukaryota eggNOG; ENOG4111Z4I; LUCA GeneTree; ENSGT00730000110951; - HOGENOM; HOG000013036; - HOVERGEN; HBG000330; - InParanoid; P01135; - KO; K08774; - OMA; LIHCCEV; - PhylomeDB; P01135; - TreeFam; TF332938; - Reactome; R-HSA-204005; COPII-mediated vesicle transport Reactome; R-HSA-5694530; Cargo concentration in the ER Reactome; R-HSA-8866910; TFAP2 (AP-2) family regulates transcription of growth factors and their receptors Reactome; R-HSA-9018519; Estrogen-dependent gene expression SignaLink; P01135; - SIGNOR; P01135; - ChiTaRS; TGFA; human EvolutionaryTrace; P01135; - GeneWiki; TGF_alpha; - GenomeRNAi; 7039; - PMAP-CutDB; P01135; - PRO; PR:P01135; - Proteomes; UP000005640; Chromosome 2 Bgee; ENSG00000163235; - CleanEx; HS_TGFA; - ExpressionAtlas; P01135; baseline and differential Genevisible; P01135; HS GO; GO:0016323; C:basolateral plasma membrane; IDA:BHF-UCL GO; GO:0009986; C:cell surface; IDA:BHF-UCL GO; GO:0031410; C:cytoplasmic vesicle; IDA:BHF-UCL GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; TAS:Reactome GO; GO:0012507; C:ER to Golgi transport vesicle membrane; TAS:Reactome GO; GO:0005615; C:extracellular space; IDA:BHF-UCL GO; GO:0000139; C:Golgi membrane; IEA:GOC GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL GO; GO:0005886; C:plasma membrane; NAS:ProtInc GO; GO:0005154; F:epidermal growth factor receptor binding; IDA:UniProtKB GO; GO:0008083; F:growth factor activity; IDA:HGNC GO; GO:0000187; P:activation of MAPK activity; IDA:HGNC GO; GO:0008283; P:cell proliferation; TAS:ProtInc GO; GO:0048208; P:COPII vesicle coating; TAS:Reactome GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:Reactome GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW GO; GO:0045741; P:positive regulation of epidermal growth factor-activated receptor activity; IDA:HGNC GO; GO:0050679; P:positive regulation of epithelial cell proliferation; IDA:HGNC GO; GO:0045840; P:positive regulation of mitotic nuclear division; IDA:HGNC GO; GO:0006357; P:regulation of transcription by RNA polymerase II; TAS:Reactome InterPro; IPR013032; EGF-like_CS InterPro; IPR000742; EGF-like_dom InterPro; IPR015497; EGF_rcpt_ligand PANTHER; PTHR10740; PTHR10740; 1 PROSITE; PS00022; EGF_1; 1 PROSITE; PS01186; EGF_2; 1 PROSITE; PS50026; EGF_3; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Disulfide bond; EGF-like domain; Glycoprotein; Growth factor; Lipoprotein; Membrane; Mitogen; Palmitate; Polymorphism; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix SIGNAL 1 23 CHAIN 24 160 Protransforming growth factor alpha /FTId=PRO_0000302744 PROPEP 24 39 Removed in mature form /FTId=PRO_0000007752 CHAIN 40 89 Transforming growth factor alpha /FTId=PRO_0000007753 PROPEP 90 160 Removed in mature form /FTId=PRO_0000007754 TOPO_DOM 24 98 Extracellular. TRANSMEM 99 124 Helical. TOPO_DOM 125 160 Cytoplasmic. DOMAIN 43 83 EGF-like. {ECO:0000255|PROSITE- ProRule:PRU00076} LIPID 153 153 S-palmitoyl cysteine LIPID 154 154 S-palmitoyl cysteine CARBOHYD 25 25 N-linked (GlcNAc...) asparagine DISULFID 47 60 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} DISULFID 55 71 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} DISULFID 73 82 {ECO:0000255|PROSITE-ProRule:PRU00076, ECO:0000269|PubMed:1632509} VAR_SEQ 32 32 Missing (in isoform 2, isoform 3 and isoform 5). {ECO:0000303|PubMed:10523832, ECO:0000303|PubMed:15489334, ECO:0000303|Ref.7} /FTId=VSP_038369 VAR_SEQ 159 160 VV -> ATLG (in isoform 3) /FTId=VSP_038370 VAR_SEQ 159 160 VV -> GCRLY (in isoform 4 and isoform 5) /FTId=VSP_038371 VARIANT 109 109 V -> M (in dbSNP:rs11466259) /FTId=VAR_024271 CONFLICT 58 58 G -> A (in Ref. 2; AAA61157) CONFLICT 65 65 Q -> H (in Ref. 2; AAA61157) CONFLICT 159 159 V -> L (in Ref. 2; AAA61157) STRAND 43 45 {ECO:0000244|PDB:1MOX} HELIX 51 54 {ECO:0000244|PDB:1YUF} STRAND 58 63 {ECO:0000244|PDB:1MOX} TURN 64 67 {ECO:0000244|PDB:1MOX} STRAND 68 73 {ECO:0000244|PDB:1MOX} STRAND 77 79 {ECO:0000244|PDB:1MOX} STRAND 84 86 {ECO:0000244|PDB:5KN5} SEQUENCE 160 AA; 17006 MW; D692184F9353DE47 CRC64; MVPSAGQLAL FALGIVLAAC QALENSTSPL SADPPVAAAV VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLAV VAASQKKQAI TALVVVSIVA LAVLIITCVL IHCCQVRKHC EWCRALICRH EKPSALLKGR TACCHSETVV
Research Areas
Citations (0)

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Background

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

References

Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35

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