Tyrosinase (Melanoma Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone T311 + OCA1/812 ]
|Application ||IHC-P, IF, FC|
|Other Accession||7299, 503555|
|Isotype||Mouse / IgG's|
|Clone Names||T311 + OCA1/812|
|Other Names||Tyrosinase, 188.8.131.52, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Tyrosinase (Melanoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.|
|Cellular Location||Melanosome membrane; Single-pass type I membrane protein|
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Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129. | Hearing, V.J., et al. 1987. Mammalian tyrosinase—the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19: 1141-1147
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