Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) Antibody
Mouse Monoclonal Antibody [Clone SPM361 ]
|Application ||IHC-P, IF, FC|
|Other Accession||7490, 591980|
|Isotype||Mouse / IgG1, kappa|
|Other Names||Wilms tumor protein, WT33, WT1|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm Isoform 4: Nucleus, nucleoplasm.|
|Tissue Location||Expressed in the kidney and a subset of hematopoietic cells|
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Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.ĀWT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Rauscher JF, Morris JF, Fredericks WJ, Lopez-Guisa J, Balakrishnan C, Jost M, Herlyn M, Rodeck U. Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms; tumor suppressor protein. Hybridoma 1998; 17:191 |
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