|Application ||IF, FC|
|Other Accession||916, 3003|
|Reactivity||Human, Mouse, Rat|
|Isotype||Mouse / IgG3, kappa|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD3 (T-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200 | Vaessen LM et al. RIV-9: a mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7 | Jiskoot W et al. Purification and stabilisation of a poorly soluble mouse IgG3 monoclonal antibody. J Immunol Methods 1991, 138(2):181-189 | Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46 | Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989 | Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. | Alberola-Ila J et al. Stimulation through the TCR/CD3 complex up-regulates the CD2 surface expression on human T lymphocytes. J Immunol 1991, 146(4):108
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.