|Application ||IHC, IF, FC|
|Other Accession||58, 59, 70, 118127, 1288, 5004836, P68032, P68133|
|Reactivity||Human, Mouse, Rat, Rabbit, Chicken, Dog, Cat|
|Isotype||Mouse / IgG1, kappa|
|Calculated MW||Multiple KDa|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Actin, Muscle Specific (Muscle Cell Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.ĀIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Tsukada T. et. al. Am J Pathol 1987, 126:51. | Tsukada T. et. al. Am J Pathol 1987, 127:389. |
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