Melanoma Marker (MART-1 + gp100 + Tyrosinase) Antibody
Mouse Monoclonal Antibody [Clone M2-7C10 + M2-9E3 + HMB45 + T311 ]
|Other Accession||2315-MART-1, 7299-Tyrosinase, 6490-gp100 (Human), 154069-MART-1, 503555-Tyrosinase, 95972-gp100 (Human), P14679-Tyrosinase, P40967-gp100 (Human)|
|Isotype||Mouse / IgG1, IgG2a & IgG2b, kappa|
|Clone Names||M2-7C10 + M2-9E3 + HMB45 + T311|
|Calculated MW||20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 90-100kDa (gp100)|
|Other Names||Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Melanoma Marker (MART-1 + gp100 + Tyrosinase) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation|
|Tissue Location||Expression is restricted to melanoma and melanocyte cell lines and retina|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.