|Application ||WB, IHC-P, IF, FC|
|Other Accession||3858, 99936|
|Isotype||Mouse / IgG2a, kappa|
|Calculated MW||56.5kDa (CK10) & 53kDa (CK13)|
|Other Names||Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin 10/13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Seen in all suprabasal cell layers including stratum corneum|
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Provided below are standard protocols that you may find useful for product applications.
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Ivanyi D et. al. Journal of Pathology, 1989, 159:7-12.,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. ,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095
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