Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody
Mouse Monoclonal Antibody [Clone SPM591 ]
|Application ||WB, IHC, IF, FC|
|Other Accession||3848 (CK1), 3852 (CK5), 3858 (CK10), 3861 (CK14), P13647 (CK5), P13645 (CK10), P02533 (CK14)|
|Reactivity||Human, Mouse, Rat|
|Isotype||Mouse / IgG1, kappa|
|Calculated MW||67kDa (CK1); 58kDa (CK5); 56.5kDa (CK10); 50kDa (CK14)|
|Other Names||Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.|
|Cellular Location||Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells|
|Tissue Location||The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis|
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This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58,2. Varma M et. al. Mod Pathol 1999;12(5):472-
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