|Application ||WB, IHC, IF, FC|
|Other Accession||3856 (CK8), 3875 (CK18), 533782, 708445 (CK8), 406013 (CK18), P05783 (CK18)|
|Isotype||Mouse / IgG's|
|Clone Names||K8.8 + DC10|
|Calculated MW||52.5kDa (CK8); 45kDa (CK18)|
|Other Names||Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin 8/18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.|
|Cellular Location||Cytoplasm. Nucleus, nucleoplasm Nucleus matrix|
|Tissue Location||Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.|
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Provided below are standard protocols that you may find useful for product applications.
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.ĀImmunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Angus B et. al. J Path, 153:377-384, 1987. | Angus B et. al. J Path, 155:71-75, 198
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