|Application ||IF, ICC, IH|
|Calculated MW||Not Known KDa|
|Other Names||Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160, GLG1, CFR1, ESL1, MG160|
|Target/Specificity||Tonsil or lymph node|
|Application Note||Immunofluorescence (0.5-1ug/ml)|
Immunocytochemistry (Acetone or paraformaldehyde fixed) (0.5-1ug/ml for 30 min)
Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT)
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Golgi Complex Antibody [Clone 371-4] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CFR1, ESL1, MG160|
|Function||Binds fibroblast growth factor and E-selectin (cell- adhesion lectin on endothelial cells mediating the binding of neutrophils).|
|Cellular Location||Golgi apparatus membrane; Single-pass type I membrane protein|
|Tissue Location||Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain.|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. It recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
1. Yuasa, K et. al. Binding and Phosphorylation of a Novel Male Germ Cell-specific cGMP-dependent Protein Kinase-anchoring Protein by cGMP-dependent Protein Kinase I. J Biol Chem, 275(7):4897-4905;2000.
2. Yoshio Endo et. al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer science 98;2007.
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