|Application ||IF, FC, IHC, E|
|Calculated MW||Unknown KDa|
|Other Names||Myeloid-associated differentiation marker, Protein SB135, MYADM|
|Target/Specificity||Tonsil or lymph node|
|Application Note||ELISA (For coating, order Ab without BSA)|
Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml)
Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Granulocyte Marker Antibody [Clone BM-2] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Membrane; Multi-pass membrane protein|
|Tissue Location||Widely expressed. Not detected in thymus.|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
de Swart RL et al. Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. PLoS Pathog 3:e178 (2007).
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