|Application ||IHC-P, IF, FC|
|Reactivity||Human, Mouse, Rat|
|Isotype||Rabbit / IgG, kappa|
|Calculated MW||89923 Da|
|Other Names||Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody|
|Application Note||Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-Ksp-Cadherin / CDH16 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Kidney specific.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
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