|Application ||WB, IHC-P, IF, FC|
|Isotype||Mouse / IgG1, kappa|
|Calculated MW||107533 Da|
|Other Names||130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)|
|Application Note||Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Western Blotting (0.5-1.0ug/ml); ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-Desmoglein-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome|
|Tissue Location||Epidermis, tongue, tonsil, esophagus and carcinomas|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
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