Anti-CELA3B / ELA3B Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, FC, E |
---|---|
Primary Accession | P08861 |
Other Accession | 728752 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse / IgG2b, kappa |
Clone Names | CELA3B/1811 |
Calculated MW | 29263 Da |
Gene ID | 23436 |
---|---|
Other Names | Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E |
Application Note | ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); ,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1.0ug/ml),Optimal dilution for a specific application should be determined. |
Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
Precautions | Anti-CELA3B / ELA3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CELA3B |
---|---|
Synonyms | ELA3B |
Function | Efficient protease with alanine specificity but only little elastolytic activity. |
Tissue Location | Pancreas. Not detectable in keratinocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.
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