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Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody

Mouse Monoclonal Antibody

     
  • IHC-P - Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody AH13572-20
    Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Monoclonal Antibody (VWF/1465)
  • WB - Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody AH13572-20
    Western Blot Analysis A) Recombinant Protein (B) human lung lysate Using Monoclonal Antibody MAb (VWF/1465)
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, IP
Primary Accession P04275
Other Accession 440848
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names VWF/1465
Calculated MW 309265 Da
Additional Information
Gene ID 7450
Other Names Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1.0ug/ml); Immunoprecipitation (0.5-1 �g/500ug protein lysate);,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-von Willebrand Factor / Factor VIII Related-Ag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VWF
Synonyms F8VWF
Function Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Cellular Location Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules
Tissue Location Plasma.
Research Areas
Citations (0)

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Background

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

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$ 199.00
$ 429.00
Cat# AH13572-20
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