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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Wilm s Tumor 1 (WT1) Antibody   

Anti-Wilm s Tumor 1 (WT1) Antibody

Recombinant Mouse Monoclonal Antibody

     
  • IHC-P - Anti-Wilm s Tumor 1 (WT1) Antibody AH13578-20
    Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm�s Tumor Recombinant Mouse Monoclonal Antibody (rWT1/857).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC
Primary Accession P19544
Other Accession 591980
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1, kappa
Clone Names rWT1/857
Calculated MW 49188 Da
Additional Information
Gene ID 7490
Other Names WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-Wilm s Tumor 1 (WT1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WT1
Function Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Cellular Location Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190) Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} Isoform 4: Nucleus, nucleoplasm
Tissue Location Expressed in the kidney and a subset of hematopoietic cells
Research Areas
Citations (0)

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Background

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

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$ 199.00
$ 429.00
Cat# AH13578-20
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