|Application ||WB, IHC-P, IF, FC|
|Isotype||Mouse / IgG1|
|Other Names||Fluorescein isothiocyanate, FITC|
|Application Note||Confocal Microscopy;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting;Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Anti-FITC (Fluorescein Isothiocyanate) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
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