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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-FITC (Fluorescein Isothiocyanate) Antibody   

Anti-FITC (Fluorescein Isothiocyanate) Antibody

Mouse Monoclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names SPM395
Additional Information
Other Names Fluorescein isothiocyanate, FITC
Application Note Confocal Microscopy;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting;Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-FITC (Fluorescein Isothiocyanate) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Background

This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

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$ 199.00
$ 429.00
Cat# AH13674-20
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