|Application ||WB, IHC|
|Other Accession||P55199, NP_006523, NM_006532|
|Reactivity||Human, Mouse, Rat, Zebrafish, Dog, Guinea Pig, Horse, Bovine|
|Predicted||Human, Mouse, Rat, Zebrafish, Chicken, Dog, Horse, Bovine|
|Calculated MW||68 kDa|
|Alias Symbol||MEN, ELL1, PPP1R68, C19orf17|
|Other Names||RNA polymerase II elongation factor ELL, Eleven-nineteen lysine-rich leukemia protein, ELL, C19orf17|
|Target/Specificity||ELL was shown to encode a previously uncharacterized elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by polymerase at multiple sites along the DNA. Functionally, ELL resembles Elongin (SIII), a transcription elongation factor regulated by the product of the von Hippel-Lindau (VHL) tumor suppressor gene.|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 100 ul of distilled water. Final anti-ELL antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||ELL antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.|
|Cellular Location||Nucleus. Nucleus speckle Nucleus, Cajal body Note=Colocalizes with EAF2 to nuclear speckles (PubMed:12446457) Colocalizes with coilin in subnuclear cajal and histone locus bodies (PubMed:12686606). Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes(PubMed:23932780).|
|Tissue Location||Expressed in all tissues tested. Highest levels found in placenta, skeletal muscle, testis and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
This is a rabbit polyclonal antibody against ELL. It was validated on Western Blot and immunohistochemistry by Abgent. At Abgent we manufacture rabbit polyclonal antibodies on a large scale (200-1000 products/month) of high throughput manner. Our antibodies are peptide based and protein family oriented. We usually provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (firstname.lastname@example.org).
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