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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   CXCR4 antibody - N-terminal region   

CXCR4 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • FC - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human HMEC-1, A549 cells using Flow Cytometry

    Sample Type: HMEC-1 and A549 cells
  • WB - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human 721_B cells using Western Blot
    CXCR4 antibody - N-terminal region (AI10012) validated by WB using 721_B cell lysate
    at 0.2-1 μg/ml.
  • WB - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human endothelial cells using Western Blot
    CXCR4 antibody - N-terminal region (AI10012) validated by WB using human microvascular endothelial cells (25ug) at 1:1000.
  • IHC - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human HMEC-1, A549 cells using Immunohistochemistry
    Immunohistochemistry with HMEC-1 and A549 cells tissue
  • IHC - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human HMEC-1, A549 cells using Immunohistochemistry
    Sample Type: human colon tissues infected ex-vivo with HIV-1
    Green: Primary
    Blue: DAPI
    Primary Dilution: 1:100
    Secondary Antibody: Donkey anti-Rabbit AF 488
    Secondary Dilution: 1:500
    Image
    Submitted by: Chiara Foglieni
    San Raffaele Scientific Institute, Milan, Italy 
  • WB - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in mouse brain extract cells using Western Blot
    WB Suggested Anti-CXCR4 Antibody
    Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti rabbit-HRP
    Secondry Antibody Dilution : 1:5,000
    Submitted by: Scott Wilson, University of Alabama at Birmingham
  • WB - CXCR4 antibody - N-terminal region AI10012
    CXCR4 antibody - N-terminal region (AI10012) in Human 721_B cells using Western Blot
    WB Suggested Anti-CXCR4 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: 721_B cell lysate
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC
Primary Accession P61073
Other Accession P61073, NP_003458, NM_003467
Reactivity Human, Mouse, Rat, Rabbit, Pig, Horse
Predicted Human, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW 40 kDa
Additional Information
Gene ID 7852
Alias Symbol CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM
Other Names C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184, CXCR4
Target/Specificity CXCR4 is a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-CXCR4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsCXCR4 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CXCR4
Function Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. Note=In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC)
Tissue Location Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
Research Areas
Citations (0)

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Background

This is a rabbit polyclonal antibody against CXCR4. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 293.00
Cat# AI10012
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