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TAF4 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - TAF4 antibody - middle region AI10506
    WB Suggested Anti-TAF4 Antibody Titration: .2-1 μg/ml
    ELISA Titer: 1:3125
    Positive Control: 293T cell lysate

    TAF4 is supported by BioGPS gene expression data to be expressed in HEK293T

    detail
  • CHIP - TAF4 antibody - middle region AI10506
    Quiescent human colon carcinoma HCT116 cultures were treated with 1% FBS for three time points (, 15, 3min) or (, 3, 6min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, CHIP
Primary Accession O00268
Other Accession NM_003185, NP_003176
Reactivity Human, Mouse, Pig, Horse, Bovine, Dog
Predicted Human, Mouse, Pig, Chicken, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 110kDa
Additional Information
Gene ID 6874
Alias Symbol FLJ41943, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Other Names Transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TBP-associated factor 4, Transcription initiation factor TFIID 130 kDa subunit, TAF(II)130, TAFII-130, TAFII130, Transcription initiation factor TFIID 135 kDa subunit, TAF(II)135, TAFII-135, TAFII135, TAF4, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-TAF4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsTAF4 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF4
Synonyms TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:10594036, PubMed:8942982). TAF4 may maintain an association between the TFIID and TFIIA complexes, while bound to the promoter, together with TBP, during PIC assembly (PubMed:33795473). Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone (PubMed:9192867).
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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References

Liu,W.L., (2008) Mol. Cell 29 (1), 81-91 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 389.00
Cat# AI10506
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