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ATP7A antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - ATP7A antibody - middle region AI10563
    WB Suggested Anti-ATP7A Antibody Titration: .2-1 μg/ml
    ELISA Titer: 1:3125
    Positive Control: HT18 cell lysate

    ATP7A is supported by BioGPS gene expression data to be expressed in HT18

    detail
  • WB - ATP7A antibody - middle region AI10563
    Lanes:
    Lane1: 4 ug mouse endothelial firboblast lysate
    Lane2: 4 ug human HUVEC lysate
    Primary Antibody Dilution:
    1:5
    Secondary Antibody:
    Anti-rabbit HRP
    Secondary Antibody Dilution:
    1:2
    Gene Name:
    ATP7A
    Submitted by:
    Anonymous
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q04656
Other Accession NM_000052, NP_000043
Reactivity Human, Mouse, Rat, Pig, Horse, Bovine, Dog
Predicted Human, Mouse, Rat, Pig, Chicken, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 163kDa
Additional Information
Gene ID 538
Alias Symbol MK, MNK, DSMAX, SMAX3
Other Names Copper-transporting ATPase 1, 3.6.3.54, Copper pump 1, Menkes disease-associated protein, ATP7A, MC1, MNK
Target/Specificity This antibody is will react to isoforms 1-5.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-ATP7A antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsATP7A antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP7A {ECO:0000303|PubMed:28389643, ECO:0000312|HGNC:HGNC:869}
Function ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity).
Cellular Location Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Melanosome membrane {ECO:0000250|UniProtKB:Q64430}; Multi-pass membrane protein. Early endosome membrane {ECO:0000250|UniProtKB:Q64430}; Multi-pass membrane protein. Cell projection, axon {ECO:0000250|UniProtKB:P70705} Cell projection, dendrite {ECO:0000250|UniProtKB:P70705}. Postsynaptic density {ECO:0000250|UniProtKB:P70705}. Note=Cycles constitutively between the TGN and the plasma membrane (PubMed:9147644). Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels. Targeting into melanosomes is regulated by BLOC-1 complex (By similarity). In response to glutamate, translocates to neuron processes with a minor fraction at extrasynaptic sites (By similarity). {ECO:0000250|UniProtKB:P70705, ECO:0000250|UniProtKB:Q64430, ECO:0000269|PubMed:9147644} [Isoform 5]: Endoplasmic reticulum
Tissue Location Widely expressed including in heart, brain, lung, muscle, kidney, pancreas, and to a lesser extent placenta (PubMed:8490646, PubMed:8490659). Expressed in fibroblasts, aortic smooth muscle cells, aortic endothelial cells and umbilical vein endothelial cells (at protein level) (PubMed:16371425)
Research Areas
Citations (0)
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References

Zhang,L.P., (2008) Chin. Med. J. 121 (2), 175-177 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 389.00
Cat# AI10563
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